Download RF-5301PC manual PDF

TitleRF-5301PC manual
TagsTypes Brochures
File Size3.0 MB
Total Pages12
Document Text Contents
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RF-5301PC

Shimadzu
Spectrof luorophotometer

C125-E008G

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World’s Highest Level Performance and WindowsTM Software

Compared to adsorbance methods, fluorescence sensitivity is tens to thousands times better — this means
that you can analyze nanogram to picogram samples with great results.
Fluorescence can be used also to identify a specific molecule in a complex background.
When the compound of interest does not exhibit natural fluorescence, functional group-specific probes may
be used to label the compound and assist your research. The synchronous scanning mode allows mixtures of
fluorochromes to be analyzed.

The personal computer directly controls the instrument for data acquisition and processing.
The WindowsTM friendly operating environment allows you to perform measurement, data processing,
editing and recording in one continuous operation with a click of the mouse. Using the Copy Graph function,
measurement data or spectra may be easily transferred to word processing or spreadsheet software for
preparation of documents or additional calculations.

The essence of fluorescence analysis is sensitivity. The high throughput optical system in the RF-5301PC
employs a blazed holographic granting, photomultiplier and digital circuit to provide the highest level S/N
ratio attainable.

From Routine Analysis To Research Employing The Highest Level Of
Sensit ivity In The World!

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Hardware
Specif ications

7

Specif ications

Light source
Excitation and emission
monochromators
Wavelength scale
Measuring wavelength range
Spectral bandwith

Wavelength accuracy
Light source compensation
Sensitivity

Wavelength scanning

Wavelength slewing speed
Response
Sensitivity selection
Interface
Dimensions and weight
Power requirements
Operational temperature range
Operational humidity range

150W Xenon lamp. Ozone resolving type lamp housing.

Concave, blazed holographic grating, F/2.5, 1300 grooves/mm.

220-990nm.

220-750nm and 0 order as standard. 220-900nm with the optional R928 photomultiplier.

6-step selection of 1.5, 3, 5, 10, 15 and 20nm.

(6nm bandwith is available for half sample height on the excitation side only.)

±1.5nm.

Dynode feedback system with monochromatic light monitoring.

The S/N ratio is 150 or higher for the Raman line of distilled water

(350nm excitation wavelength, 5nm spectral band width, and 2 second response for 98% of the full scale).

7-step selection of Survey (about 5500nm/min), Super (about 3000nm/min)

Very Fast, Fast, Medium, Slow and Very Slow.

About 20,000nm/min.

8-step selection of 0.02, 0.03, 0.1, 0.25, 0.5, 2, 4 and 8 seconds for 98% of the full scale.

2-steps of HIGH and LOW. (The sensitivity at HIGH is about 50 times that of LOW.)

RS-223C interface, interface for autosampler, and interface for sipper unit.

667W x 530D x 270H mm; 43kg.

100, 120, 220, 240V; 50/60Hz; 400VA.

15-35°C

40-80% (Below 70% with temperature higher than 30°C).

Software
Specif ications

Measurement

Data processing

Filing
Data output

Maintenance
User interface
Other functions
PC requirements

Excitation, emission and synchronous spectrum measurement, time-course measurement,

quantitation, automatic search of optimal excitation and emission wavelengths, PopUp ScanTM.

Arithmetic calculation between spectrum and a constant, smoothing, 1st through

4th derivatives, 1/Y, logarithmic conversion, data printout (with or without activity

value computation), peak pick, point pick, area calculation, averaging

(in quantitation), generation of calibration curves of 1st through 3rd order.

Save, recall, and delete, of data. Conversion into ASCll and DIF formats.

Automatic scale adjustment, readout of data at user-specified point, data printout

(preview function provided), selection of colors and tyoes of curves.

Automatic monitoring of signal-to-noise ratio, monitoring of the run time of light source lamp.

SpeedBoxTM (assign icons for commonly used menu commands).

Data exchange via clipboard, auto response control, automatic shutter.

IBM-PC/AT or 100% compatible; i486 or higher CPU; 8 Mbyte or larger main

memory. Operates on MS-Windows version 3.1 or higher.

Windows is a registered trademark of Microsoft Corp. SpeedBox and PopUp Scan are registered trademarks of Shimadzu Scientific Instruments,

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Cat, No. 204-04691

Includes 8 filters: two broad-band
interference filters U-340 and
B-390 for isolation of excitation
wavelengths of 300-380 and 350-
450nm, respectively. Six cutoff
filters: UV-29, L-41, Y-46, Y-50,
O-56 and R-60 with approximately
50%T at 300, 420, 460, 500, 560
and 600nm, respectively.

Filter Set

Cat, No. 204-07550

Cat, No. 204-03285-04

Used to correct excitation and
emission spectra in the wavelength
region from 250nm to 600nm, using
rhodamine solution as the reference
(quantum counter).

Spectra Correction
Accessory

Used for fluorescence monitoring of
effluents in column chromatography.
Made of fused silica. Requires
adapter 206-83934-91

Rectangular Flow Cell ,
120µL

Cat, No. 204-09100

Builds an automatic multi sample
spectrophotometry system in
combination with a sipper unit.
Up to 100 test tubes are set
together on the rack. The aspirating
nozzle is programmable to move X,
Y and Z (vertical) direction.

ASC-5 Auto Sample
Changer

Cat, No. 208-97263

NTT-2200P Constant-
Temperature Water
Circulator

Circulates temperature controlled
water to a constant-temperature cell
holder.
-Temperature range: Ambient + 5°C to + 80°C
-Temperature control precision: ±0.05°C~
-Max. pumping rate: 27/31 L/min,
9.5/13m (50/60Hz)
-External circulation nozzle: 10.5mm
OD (both outlet and return)
-Tank capacity: About 10L (9L during use)
-Safety feature: Detection of over-
temperature of Upper or Lower
limits, Detection of heater wire
malfunction, Protection of heating
too few circulating water, Detection
of sensor malfunction, Independent
over heat protection, Over current
circuit protector

-Standard accessories: Lid with
handles, Rubber hose (4m, 9mm
ID), Hose clamps (4pc.), Instruction
manual

-Dimensions: 270W x 560H x 400D (mm)
-Power requirements: 100VAC,
1250VA, with 1.7m power cord and
grounded plug
Note: Piping to Constant-
Temperature Cell Holder (No.206-
88950-**) is possible by the hose
(9mm ID) of accessories.

Cat, No. 206-83000-91

Aspirates solution samples, using a
peristaltic pump driven by a
stepping motor, to be measured
successively. Minimum sample
volume is 2mL, Linked operation
with the ASC-5 Auto Sampler
possible.

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SHIMADZU CORPORATION. International Marketing Division
3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan Phone: 81(3)3219-5641 Fax. 81(3)3219-5710
Cable Add.:SHIMADZU TOKYO

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7102 Riverwood Drive, Columbia, Maryland 21046, U.S.A.
Phone: 1(410)381-1227 Fax. 1(410)381-1222 Toll Free: 1(800)477-1227

SHIMADZU EUROPA GmbH
Albert-Hahn-Strasse 6-10, 47269 Duisburg, F.R. Germany Phone: 49(203)7687-0 Fax. 49(203)766625

SHIMADZU (ASIA PACIFIC) PTE LTD.
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Phone: 65-6778-6280 Fax. 65-6779-2935

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Units F, 10-16 South Street Rydalmere N.S.W. 2116, Australia
Phone: 61(2)9684-4200 Fax. 61(2)9684-4055

SHIMADZU DO BRASIL COMÉRCIO LTDA.
Avenida Marquês de São Vicente, 1771. Barra Funda CEP : 01139-003-São Paulo-SP, Brasil
Phone: (55)11-3611-1688 Fax. (55)11-3611-2209

SHIMADZU (HONG KONG) LIMITED
Suite 1028 Ocean Center, Harbour City, Tsim Sha Tsui, Kowloon HONG KONG
Phone: (852)2375-4979 Fax. (852)2199-7438

SHIMADZU INTERNATIONAL TRADING (SHANGHAI) Co., LTD. SHANGHAI OFFICE
24th Floor, Shanghai Xin Hualian Building, No.755 Huaihai Zhong Lu, Shanghai, China
Phone: 86-21-6472-8442 Fax. 86-21-6472-8648

Overseas Offices
Istanbul, Moscow

URL http://www.shimadzu.com

The RF-1501 simply outperforms every other
instrument in its class.
High sensitivity gives you the ability to measure
very small samples and detect trace concentrations.
Compact design and availability of inexpensive
program packs for kinetics, intracellular calcium
and time course make it easily expandable.
Other options include software for PC
management.

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